a.       Gel Retardation Assay Cre-recombinase recombine a pair of short target sequence called Lox sequences, hence the name. M13 is a virus which infects E. coli. Tell us all about it in this ultimate trivia quiz on biotechnology! c.       RFLP c.       pUC 19 d.      Homologous recombination, (7). Alec Jeffery’s name is associated with: Imidazole is used for the purification of His-tagged (Histidine-tagged) proteins using affinity chromatography. d.      0.25 N HCl. d.      7 X 107, (2). The Histidine bound to the Nickel ion column and the other impurities will elute out. (a). MCQ 08 – Biotechnology Questions from CSIR Papers – Set 2, @. The name Kary Mullis is associated with: Cre-Lox recombination: is a site-specific recombination technique to induce large-scale mutations in specific regions of DNA by deletion, insertion or translocations. Title Test Test Answer Key Worksheet Answer Key L. A. Detective L.A. Detective - Test Sheet L.A. Detective - Test Answer Key L.A. Detective - Worksheet Answer Key The Lost Ship The Lost Ship - Test Sheet The Lost Ship - Test part1, Page 1 QUIZ : Biotechnology QUESTIONS AND ANSWERS b.      Temin and Baltimore c.       Tatum and Lederberg Gel Retardation Assay is better known as Electrophoretic Mobility Shift Assay or EMSA. b. The His-tagged proteins were first run through a Nickel ion column. c.       6X SSC These 3 … The plasmid also possesses a unique multiple cloning regions with restriction sites for more than 40 restriction enzymes. collect cheek cells, burst cells to release DNA, separate DNA from proteins and debris, isolate b.      Creatine phosphatase Which of the following is a dual resistance vector? Answer: Endosperm. The antisense gene involved in the production of Flavr Savr tomato is : In RFLP analysis, the variations of the fragments of DNA obtained after treating with a specific endonuclease enzyme is analyzed for its polymorphism. b.      Lactamase Site-directed mutagenesis: a molecular method to induce specific sequence changes in a gene or gene product to investigate the importance of that particular gene in the organism. MCQ 01 – Introduction to Biotechnology, @. It is the first constructed cloning vector, created in 1977 in the laboratory of Herbert Boyer. pUC18 and pUC19 are also cloning vectors. a.       Imidazole EMSA is an electrophoresis technique to detect protein-DNA or protein RNA interactions. What do you know about the subject? c.       DNA recombinase Tell us all about it in this ultimate trivia quiz on biotechnology! b.      Beta-carotene d.      PCR, (4). MCQ 04 – Recombinant DNA (rDNA) Technology, @. The vector was named after the researchers who constructed it for the first time. Answer Key with Explanations 1. c.       Two point crossovers His-tagged proteins can be eluted from Ni columns using: O Level Biology Multiple Choice Questions and Answers (MCQs): Quizzes & Practice Tests with Answer Key provides course review tests for competitive exams to solve 1825 MCQs. The technique of DNA fingerprinting was developed by Alec Jeffreys in 1984. 2 X 107 The human genome contains approximately 3 billion base pairs located on 23 chromosomes in its haploid set. d.      pBR 322, (3). 12. Golden rice is genetically modified rice which produces beta-carotene in their grains. d.      Site-directed mutagenesis, (5). ‘p’ stands for ‘plasmid’, ‘B’ stands for Bolivar and ‘R’ stands for Rodriquez. Start studying H Bio: Biotechnology: DNA extraction. Chain Termination Reaction b. a.       Domains of transcription factors

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